Identification of key genes in breast cancer cell line under hypoxia condition: A bioinformatics analysis

Document Type: Original Article

Authors

1 Department of Medical Genetics and Molecular Biology, Faculty of Medicine, Iran University of Medical Sciences (IUMS),Tehran, Iran

2 Department of Medical Immunology, Faculty of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran

3 Department of Dentistry, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Canada

4 Department of Medical Genetics and Molecular Biology, Faculty of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran

10.22631/ijbmph.2019.186711.1111

Abstract

Background. The present study attempted to investigate the key pathways and genes which are associated with hypoxia in the human breast carcinoma cell line MDA-MB-231 with searched in gene expression omnibus (GEO) database for mRNA microarray data of MDA-MB-231 in normal and hypoxia condition.
Methods. Three GEO datasets GSE37340, GSE39042, and GSE42416 were downloaded from the Gene Expression Omnibus (GEO) database that these GEO profiles have of 9 cell lines in hypoxia condition and 8 cell lines in normal condition. The differentially expressed genes (DEGs) between MDA-MB-231 cell line in hypoxia and normal condition were analyzed by Geo2R software. Next, all the differentially expressed genes (DEGs) with p Results. 34 genes were found to be at least two datasets (i.e., SLC2A3, BNIP3, ENO2, PFKFB3, PLOD2, SLC2A1, HK2, ADM and etc.) that two genes among up regulated genes (HK2, ADM) were expressed in all three datasets.
Conclusion. These identified genes and pathways could help to understand the mechanism of development of(Triple-negative breast cancer) TNBC under hypoxia condition. Also HK2, ADM, CENP family, might be promising targets for the TNBC treatment.

Keywords