Anticoagulant and cytotoxic assessment of L-amino acid oxidase from Indian cobra (Naja naja) venom

Document Type: Original Article

Authors

1 Department of Biotechnology, JSS Science & Technology university

2 Department of Biochemistry, JSS University, Mysore

3 Department of Biotechnology, JSS Science & technology university

4 Deaprtment of Chemistry, University of Mysore

5 Department of Biotechnology, JSS Science & Technology

Abstract

 
Introduction: Snake venom L-amino acid oxidase (SV-LAAO) has promising therapeutic prospects because of its effects on various biological functions. Purified Naja naja venom enzyme L-amino acid oxidase (NnLAAO) was studied to examine its anticoagulant and cytotoxic properties.
Methods: Activated partial prothrombin time (APPT) assay and prothrombin time (PT) both of these assays are used to access the anti-coagulant property of NnLAAO on human blood sample. Sulforhodamine B (SRB) protocol is performed on MDA MB-231and HCT 116 cell, which are treated with different doses (0.4µg-100µg) of NnLAAO for 48h incubation time, respectively, and the percentage of inhibition was compared with oxaloplatin as positive control.
Results: NnLAAO significantly prolonged the APPT and showed a negligible effect on the PT. NnLAAO inhibited cytotoxicity in a dose-dependent manner in both breast cancer MDA MB-231 and colon adenocarcinoma (HCT-116) cell lines with 60% and 78% of inhibition respectively.
Conclusion: The NnLAAO enzyme of Indian cobra venom is a promising anticoagulant as well an anticancer agent for various cell lines because of its efficiency and specificity, as it will offer better treatment and response with fewer side effects.

Keywords



Volume 2, Issue 1
Winter 2019
Pages 11-15
  • Receive Date: 03 August 2018
  • Revise Date: 18 August 2018
  • Accept Date: 18 August 2018