TY - JOUR ID - 100342 TI - Synergistic antibacterial effects of methanolic extract of Melissa officinalis L. and Mouthwash Vi-one on Streptococcus mutant and Streptococcus sanguinis JO - International Journal of Biomedicine and Public Health JA - IJBMPH LA - en SN - AU - Faraji, Ali Asghar AU - Issazadeh, Khosro AU - Rouhi, Samaneh AU - Zaboli, Fatemeh AU - Khasi, Bita AU - Ebnabbas, Jalileh AD - Department of Biology, Faculty of Agriculture and Food Industry, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran AD - Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Lahijan Branch, Lahijan, Iran AD - Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran AD - Social Development and Health Promotion Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran AD - Lung Diseases and Allergy Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran Y1 - 2019 PY - 2019 VL - 2 IS - 4 SP - 57 EP - 60 KW - Antibacterial Effect KW - Melissa officinalis L KW - mouthwash KW - Streptococcus mutant DO - 10.22631/ijbmph.2018.132744.1057 N2 - Introduction: The use of chemical and vegetable compounds reduces the microbial plaque of the tooth. The aim of this study was to investigate the synergistic antibacterial effects of methanolic extract of Melissa officinalis L. (Lemon balm)and mouthwash Vi-one on Streptococcus mutant and Streptococcus sanguinis. Methods: Methanolic extract of lemon balm was prepared by Soxhlet method. The concentrations of 250, 125, 62.5, 31.25, 15.26, 7.81, 3.9, 1.95, 0.97 and 0.48 mg/ml of methanol extract and mouthwash prepared and mixed in the same proportion.Agar well diffusion, minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were used to determine the antibacterial effect. Results: The highest non-growth zones were 21 mm for Streptococcus mutant and 22 mm for Streptococcus sanguinis which observed at a concentration of 250 mg/ml. The best value of MIC and the MBC for both bacteria were 7.81 and 62.5 mg/ml, respectively. As the concentration increased, antibacterial activity increased as well (p≤0.05). Conclusion: The results of this study showed the synergistic antibacterial effects of lemon balmand mouthwash on bacteria. More in vivo researches are needed to confirm and use the above combination. UR - https://www.ijbmph.com/article_100342.html L1 - https://www.ijbmph.com/article_100342_dbcd432cc38c5244dfc596985974af14.pdf ER -